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These qualitative results can be combined with quantitative results into a single report.

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A Agilent ChemStation for LC 3D Systems Understanding Your Spectra Module Understanding Your ChemStation Spectra Module Notices Agilent Technologies, Inc.

No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc.

Microsoft is a U.S.

Microsoft Corporation.

Manual Part NumberG2180-90024 Edition0608 Printed in Germany Agilent Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn, Germany Software RevisionThis guide is valid for B.04.xx revisions of the Agilent ChemStation for LC 3D Systems software, where xx refers to minor revisions of the software that do not affect the techni-cal accuracy of this guide.

Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose.

Agilent shall not be liable for errors or for incidental or consequential damages in connec-tion with the furnishing, use, or per-formance of this document or of any information contained herein.

Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall control.

Technology Licenses The hardware andor software described in this document are furnished under a license and may be used or copied only in accor-dance with the terms of such license.

Restricted Rights LegendIf software is for use in the performance of a U.S.

Government prime contract or subcon-tract, Software is delivered and licensed as Commercial computer software as defined in DFAR 252.227-7014 (June 1995), or as a commercial item as defined in FAR 2.101(a) or as Restricted computer soft-ware as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause.

It calls attention to an operat-ing procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data.

Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in personal injury or death.

Do not proceed beyond a WARNING notice until the indicated condi-tions are fully understood and met.

Understanding Your ChemStation Spectra Module 3 In This GuideThis handbook describes the concepts behind the spectra module of the Agilent ChemStation revision B.04.0x.

It complements the information in the Understanding Your ChemStation manual with the special spectra concepts applicable to the Agilent ChemStation for LC 3D, CE systems, and the UV-visible part of the Agilent ChemStation for LC-MSD.

For details of installation and configuration of your spectra module with an Agilent ChemStation for LC 2D system, see the Installing Your ChemStation manual.

Understanding Spectral Analysis This chapter covers the following: What is spectral analysis Determining the optimum detection wavelength.

Spectral report styles.

Spectral Libraries Traditional data systems quantify the detected compounds by correlations based on retention times.

This can lead to erroneous identification, if: compounds other than the calibrated ones appear in the specified retention time window, more than one peak appears in the specified retention time window, or the compound is retained on the column longer than the specified window because of solvent flow rate changes or, more commonly, changing column characteristics.

One way to avoid such errors is to use peak qualifiers as described in the Understanding your Agilent ChemStation manual.

With a diode array detector such errors can be avoided, since UV-visible spectra can be used to confirm a peaks identity.

Standard spectra are acquired from a reference sample under well-defined chromatographic conditions and stored in a database (spectral library).

Spectra can be overlaid for visual comparison and the similarity of standard and sample spectra can be calculated.

Understanding Your ChemStation Spectra Module The ChemStation can automate this process for all peaks within a chromatogram and for all chromatograms within a sequence.

In addition to such peak confirmation, the software can perform a peak purity check, using the settings in the Purity Preferences dialog box.

Agilent Chemstation Software Download 0 SHARE

Agilent Chemstation Software Manual